R&D Systems Recombinant Mouse IFN-gamma R2 Protein, CF, 50 ug
RDS1185GR050
This is a regulated item, so additional permits may be required which may lead to an extended ETA. Please Contact us at lifescience@invitro.com.au for more information.
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse IFN-gamma R2 protein
Met1-Val243, with a C-terminal 6-His tag
Met1-Val243, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser21
Predicted Molecular Mass
26 kDa (monomer)
SDS-PAGE
37-50 kDa, reducing conditions and visualized by silver stain.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IFN-gamma R1 Fc Chimera (Catalog # 1026-GR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse IFN‑ gamma R2 in the presence of Recombinant Mouse IFN-gamma (Catalog # 485-MI). The concentration of Recombinant Mouse IFN‑ gamma R2 that produces 50% of the optimal binding response was found to be approximately 0.8-4 μg/mL.
When Recombinant Mouse IFN-gamma R1 Fc Chimera (Catalog # 1026-GR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Mouse IFN‑ gamma R2 in the presence of Recombinant Mouse IFN-gamma (Catalog # 485-MI). The concentration of Recombinant Mouse IFN‑ gamma R2 that produces 50% of the optimal binding response was found to be approximately 0.8-4 μg/mL.
Formulation, Preparation and Storage
1185-GR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 250 μg/mL in PBS.
Reconstitution Buffer Available: RB01: Reconstitution Buffer 1 (PBS) 2 mL |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|